Induction of experimental mammary carcinogenesis in rats with 7,12-dimethylbenz(a)anthracene.

Revista do Hospital das Clinicas Pub Date : 2004-10-01 Epub Date: 2004-10-29 DOI:10.1590/s0041-87812004000500006

Alfredo Carlos S D Barros, Elisa Naomi K Muranaka, Lincon Jo Mori, Christina Helena T Pelizon, Kyoshi Iriya, Giovana Giocondo, José Aristodemo Pinotti

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引用次数: 96

Abstract

Purpose: To test an experimental model of chemical mammary carcinogenesis induction in rats.

Methods: Twenty young virgin Sprague-Dawley female rats, aged 47 days, received 20 mg of 7,12-dimethylbenz(a)anthracene (DMBA) intragastrically by gavage. Afterwards, at 8 and 13 weeks, their mammary glands were examined. At the end of the experiment, the animals were sacrificed, and the mammary tumors were measured and weighed. Tumor fragments were analyzed using light microscopy.

Results: Eight weeks after DMBA injection, 16 rats presented at least 1 breast tumor (80%). After 13 weeks, all of them (100%) developed breast carcinomas that were confirmed by histopathological analysis.

Conclusion: This experimental animal model of chemical mammary induced carcinogenesis is feasible and can be used in further experiments on the role of tumorigenic biomodulator substances.

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7,12-二甲基苯(a)蒽对大鼠实验性乳腺癌的诱导作用。

目的:建立化学诱导大鼠乳腺癌的实验模型。方法:20只47日龄的年轻雌性sd大鼠灌胃7,12-二甲基苯(a)蒽(DMBA) 20 mg。随后，在第8周和第13周，检查了它们的乳腺。实验结束时，处死动物，对乳腺肿瘤进行测量和称重。光镜下分析肿瘤碎片。结果:注射DMBA后8周，16只大鼠出现至少1个乳腺肿瘤(80%)。13周后，所有患者(100%)发展为乳腺癌，经组织病理学分析证实。结论:建立的化学乳腺致癌实验动物模型是可行的，可用于进一步开展致瘤性生物调节剂作用的实验。

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Revista do Hospital das Clinicas

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