Evaluation of DNA damage in lymphocytes of cardiologists exposed to radiation during cardiac catheterization by the COMET ASSAY.

Bulent Boyaci, Ridvan Yalçin, Atiye Cengel, Onur Erdem, Ovsev Dörtlemez, Halis Dörtlemez, Semra Sardas
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引用次数: 15

Abstract

This study was designed to evaluate the possible in vivo induction of DNA damage by exposure to radiation in cardiologists. The alkaline comet assay (single cell gel electrophoresis, SCGE), which appears to be a promising tool with which to estimate DNA damage at the single cell level, has been used. The assay was carried out on 30 cardiologists currently employed in a busy cardiac service and 30 healthy unexposed controls. Venous blood samples were obtained from the exposed and control subjects and SCGE was examined in 100 cells graded as undamaged, intermediate, and tailed nuclei. The number of undamaged nuclei was almost the same in control and exposed subjects. The extent of DNA migration (SCGE assay) did not distinguish between the samples in either the nonsmoker exposed or nonsmoker control subjects, which leads one to wonder whether a difference in DNA damage really exists. Previous studies reported increased DNA damage in blood lymphocytes of smokers. In our study, the percentage of damaged cells increased either with the frequency of smoking or exposure to radiation. A statistically significant difference was observed both in smokers and exposed subjects. In conclusion, the elevated grade of DNA damage in cardiologists exposed to radiation indicates a possible genotoxic hazard, therefore, careful measures and full cooperation between cardiologists and radiologists should be undertaken to reduce the exposure to radiation.

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用彗星测定法评估心导管插管期间暴露于辐射的心脏病专家淋巴细胞的DNA损伤。
本研究旨在评估暴露于辐射对心脏病专家体内DNA损伤的可能诱导。碱性彗星试验(单细胞凝胶电泳,SCGE),这似乎是一个有前途的工具,估计DNA损伤在单细胞水平,已被使用。该试验在30名目前在繁忙的心脏服务部门工作的心脏病专家和30名健康未暴露的对照组中进行。从暴露者和对照组获得静脉血样本,并在100个细胞中进行SCGE检测,这些细胞分为未损伤核、中间核和尾核。未损伤核的数量在对照组和暴露组几乎相同。DNA迁移的程度(SCGE测定)并没有区分非吸烟者和非吸烟者对照组的样本,这让人们怀疑DNA损伤是否真的存在差异。先前的研究报告了吸烟者血液淋巴细胞DNA损伤的增加。在我们的研究中,受损细胞的百分比随着吸烟或暴露于辐射的频率而增加。在吸烟者和暴露者中都观察到统计学上的显著差异。总之,暴露于辐射的心脏病专家DNA损伤程度升高表明可能存在遗传毒性危害,因此,应采取谨慎措施并在心脏病专家和放射科医生之间进行充分合作,以减少辐射暴露。
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