A genetic locus of Helicobacter pylori inversely associated with gastric intestinal metaplasia

FEMS immunology and medical microbiology Pub Date : 2005-05-01 DOI:10.1016/j.femsim.2005.02.003

Quanjiang Dong , Maria O’Sullivan , Abdurrazag Nami , Paul Dowling , Gwen Murphy , Martin Buckley , Colm O’Morain

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引用次数: 2

Abstract

The genomic contents of Helicobacter pylori strain C1 from a patient with gastric cancer and strain 98587 from a patient with duodenal ulcer disease were compared using a rapid subtractive hybridisation approach. A total of 11 tester-specific sequences representing gene specificity, DNA rearrangement and sequence variation were identified. This included two novel sequences, clone P32 and clone F5, which have no significant homologue in the database. H. pylori strains positive for clone P32 were less prevalent in patients with gastric intestinal metaplasia (12.5%) than in duodenal ulcer (39.1%) (p = 0.036), or chronic gastritis (38.1%) (p = 0.036). The results suggest that H. pylori clone P32 is potentially a useful marker for distinguishing intestinal metaplasia associated strains from others.

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幽门螺杆菌基因位点与胃肠化生呈负相关

采用快速减法杂交方法比较了胃癌患者幽门螺杆菌C1菌株和十二指肠溃疡患者幽门螺杆菌98587菌株的基因组含量。共鉴定出11个具有基因特异性、DNA重排和序列变异的测试者特异性序列。这包括两个新的序列，克隆P32和克隆F5，在数据库中没有明显的同源性。P32克隆阳性幽门螺杆菌在胃肠道化生(12.5%)患者中的感染率低于十二指肠溃疡(39.1%)(p= 0.036)和慢性胃炎(38.1%)(p= 0.036)。结果表明，幽门螺杆菌克隆P32可能是区分肠化生相关菌株的有用标记。

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来源期刊

FEMS immunology and medical microbiology 医学-传染病学

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3-8 weeks