Class 1 integrons in Pseudomonas aeruginosa isolates from clinical settings in Amazon region, Brazil

FEMS immunology and medical microbiology Pub Date : 2005-06-01 DOI:10.1016/j.femsim.2005.01.004

Érica L. Fonseca , Verônica V. Vieira , Rosângela Cipriano , Ana C.P. Vicente

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引用次数: 88

Abstract

A hundred and six Pseudomonas aeruginosa isolates from clinical cases were screened using PCR for the presence of integrons and associated resistance gene cassettes. Forty-four isolates harboured class 1 integrons (41.5%), of which 29 isolates (66%) also carried gene cassettes. The aacA gene was most frequently found within class 1 integrons (69%), followed by bla_OXA family genes (52%). From class 1 integron-positive strains, we detected a total of 15 isolates (34%) carrying no gene cassettes. Restriction fragment-length polymorphism analysis of the integrons variable region revealed some identical structures, as well as distinct profiles indicating heterogeneity among these cassette regions. Multiresistance was observed in 71% of isolates, nevertheless no strong correlation was observed between integron presence and multiresistance. This is the first report showing class 1 integron prevalence and gene cassette content in P. aeruginosa isolates from clinical settings in the Brazilian Amazon.

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巴西亚马逊地区临床环境中铜绿假单胞菌分离物中的1类整合子

采用PCR技术对临床病例分离的106株铜绿假单胞菌进行整合子及相关耐药基因盒的筛选。含1类整合子44株(41.5%)，其中29株(66%)携带基因盒。aacA基因在1类整合子中最常见(69%)，其次是blaOXA家族基因(52%)。从1类整合子阳性菌株中，我们共检测到15株(34%)不携带基因盒。对整合子可变区进行限制性片段长度多态性分析，发现整合子可变区有一些相同的结构，但也有不同的分布，表明这些盒式区域具有异质性。在71%的分离株中观察到多重耐药，但整合子的存在与多重耐药之间没有很强的相关性。这是第一份显示巴西亚马逊地区临床环境中铜绿假单胞菌1类整合子流行率和基因盒含量的报告。

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FEMS immunology and medical microbiology 医学-传染病学

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3-8 weeks