Phenotypic and genotypic discrepancy of Streptococcus pneumoniae strains isolated from Asian countries

FEMS immunology and medical microbiology Pub Date : 2005-07-01 DOI:10.1016/j.femsim.2005.01.012

Kwan Soo Ko , Won Sup Oh , Kyong Ran Peck , Jang Ho Lee , Nam Yong Lee , Jae-Hoon Song

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引用次数: 17

Abstract

Non-typeable isolates of Streptococcus pneumoniae collected from Asian countries were characterized by optochin susceptibility test, bile solubility test, multilocus sequence typing of housekeeping genes, amplification of virulence-related genes, 16S rDNA-RsaI digestion, and 16S rDNA sequencing. Six of 54 non-typeable pneumococcal isolates showed divergence of gene sequences of recP and xpt from typical pneumococcal strains. Of these six atypical pneumococcal strains, two showed different results in optochin susceptibility or bile solubility test from typical pneumococcal strains. All six isolates showed high sequence dissimilarities of multilocus sequence typing, 16S rDNA sequences, and lytA sequences from typical S. pneumoniae strains. Data from this study suggest that classic tests such as optochin susceptibility and bile solubility tests may lead to incorrect identification of S. pneumoniae. These atypical strains may belong to different bacterial species from S. pneumoniae.

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亚洲国家肺炎链球菌分离株的表型和基因型差异

对收集自亚洲国家的不可分型肺炎链球菌进行光敏试验、胆汁溶解度试验、管理基因多位点序列分型、毒力相关基因扩增、16S rDNA- rsai酶切和16S rDNA测序。54株非分型肺炎球菌分离株中有6株与典型肺炎球菌菌株的recP和xpt基因序列存在差异。在这6株非典型肺炎球菌中，有2株在光素敏感性或胆汁溶解度试验中表现出与典型肺炎球菌不同的结果。6株分离株在多位点序列分型、16S rDNA序列和lytA序列上均与典型肺炎链球菌具有较高的序列差异性。本研究的数据表明，经典的检测方法，如光chin药敏试验和胆汁溶解度试验，可能导致肺炎链球菌的错误鉴定。这些非典型菌株可能属于与肺炎链球菌不同的细菌种类。

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FEMS immunology and medical microbiology 医学-传染病学

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3-8 weeks