Coccidioidomycosis and blastomycosis: Advances in molecular diagnosis

Ralf Bialek , Gloria M. González , Dominik Begerow , Ulrike E. Zelck
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引用次数: 40

Abstract

Clinical isolates of Coccidioides spp. and Blastomyces dermatitidis can be identified by chemiluminescent DNA probes and PCR assays targeting multicopy genes. In fixed tissue samples, cells of the two fungi are specified by in situ hybridization and PCR assays targeting 18S rDNA but sequencing of the products is mandatory. Nested PCR assays targeting genes encoding species- or genus-specific proteins like proline rich antigen of Coccidioides spp. and B. dermatitidis adhesin facilitate amplification of specific DNA from fixed tissue samples. The value of DNA amplification from native specimens of suspected cases of coccidioidomycosis or blastomycosis still needs to be determined.

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球孢子菌病和芽孢菌病:分子诊断的进展
利用化学发光DNA探针和多拷贝基因PCR技术对临床分离的球虫和皮炎芽孢菌进行了鉴定。在固定组织样本中,这两种真菌的细胞通过靶向18S rDNA的原位杂交和PCR测定来指定,但产物的测序是强制性的。巢式PCR检测针对编码种或属特异性蛋白质的基因,如球虫的脯氨酸丰富抗原和皮炎B.粘附素,有助于从固定组织样本中扩增特异性DNA。疑似球孢子菌病或芽生菌病本地标本的DNA扩增值仍有待确定。
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