[Mapping of apple Co gene using SSR markers].

Shi yan sheng wu xue bao Pub Date : 2005-06-01
Yi Ke Tian, Cai Hong Wang, Hong Yi Dai
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引用次数: 0

Abstract

Columnar apple is an important genetic resource for tree form breeding of apple. In this study, 106 individuals of the F1 population derived from 'Spur Fuji' (coco)x 'Telamon'(Coco) were used as plant materials for screening SSR markers linked to gene. By bulked segregating analysis (BSA), eight SSR markers from the tenth linkage group of apple genome were tested. Finally, three of them, COL, CH02a10 and CH03d11, were identified to be SSR markers of Co gene. Linkage analysis showed that the genetic distance of COL, CH02a10 and CH03d11 to Co locus was 15.3cM, 22.2cM and 3.9 cM, respectively. On the linkage map of these markers, Co gene was located between COL and CH03d11.

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利用SSR标记定位苹果Co基因。
柱状苹果是苹果树形育种的重要遗传资源。本研究以‘Spur Fuji’(coco)x‘Telamon’(coco) F1群体的106个个体为材料,筛选了与基因相关的SSR标记。采用散装分离分析(BSA)对苹果基因组第10连锁群中的8个SSR标记进行了检测。最后,鉴定出COL、CH02a10和CH03d11为Co基因的SSR标记。连锁分析表明,COL、CH02a10和CH03d11与Co位点的遗传距离分别为15.3cM、22.2cM和3.9 cM。在这些标记的连锁图谱上,Co基因位于COL和CH03d11之间。
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