Analysis and expression of STE13ca gene encoding a putative X-prolyl dipeptidyl aminopeptidase from Candida albicans

FEMS immunology and medical microbiology Pub Date : 2005-09-01 DOI:10.1016/j.femsim.2005.05.020

Consuelo Bautista-Muñoz, César Hernández-Rodrı́guez, Lourdes Villa-Tanaca

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引用次数: 12

Abstract

Candida albicans STE13ca gene was identified by its homology to the Saccharomyces cerevisiae STE13 gene that encodes for the dipeptidyl aminopeptidase A (DAP A) involved in the maturation of α-factor mating pheromone. Our study revealed that C. albicans ATCC 10231 depicts dipeptidyl aminopeptidase activity. We also analyzed the expression of the STE13ca gene homologue from this pathogenic yeast. This gene of 2793 pb is homozygotic and encodes for a predicted protein of 930 amino acids with a molecular weight of 107,035 Da. The predicted protein displays significant sequence similarity to S. cerevisiae Ste13p. This C. albicans gene is located in chromosome R. STE13ca gene increases its levels of expression in conditions of nutritional stress (proline as nitrogen source) and during formation of the germinal tube, suggesting a basic biological function for the STE13ca in this yeast.

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白色念珠菌x -脯氨酸二肽基氨基肽酶编码基因STE13ca的分析与表达

白念珠菌STE13ca基因与酿酒酵母STE13基因同源，后者编码参与α-因子交配信息素成熟的二肽基氨基肽酶A (DAP A)。我们的研究表明，白色念珠菌ATCC 10231具有二肽基氨基肽酶活性。我们还分析了该致病酵母中STE13ca基因同源物的表达。该基因全长2793 pb，是纯合子基因，可编码一个分子量为107,035 Da的930个氨基酸的蛋白质。预测的蛋白序列与酿酒葡萄球菌Ste13p具有显著的相似性。STE13ca基因在营养胁迫条件下(脯氨酸为氮源)和生发管形成过程中表达水平增加，表明STE13ca基因在该酵母中具有基本的生物学功能。

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来源期刊

FEMS immunology and medical microbiology 医学-传染病学

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3-8 weeks