Cristina Elena Canteros , María Fernanda Zuiani , Viviana Ritacco , Diego E. Perrotta , María Rocío Reyes-Montes , Julio Granados , Gerardo Zúñiga , Maria Lucia Taylor , Graciela Davel
{"title":"Electrophoresis karyotype and chromosome-length polymorphism of Histoplasma capsulatum clinical isolates from Latin America","authors":"Cristina Elena Canteros , María Fernanda Zuiani , Viviana Ritacco , Diego E. Perrotta , María Rocío Reyes-Montes , Julio Granados , Gerardo Zúñiga , Maria Lucia Taylor , Graciela Davel","doi":"10.1016/j.femsim.2005.05.015","DOIUrl":null,"url":null,"abstract":"<div><p>Intact chromosomes of 19 clinical isolates of <span><em>Histoplasma capsulatum</em></span> recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5–7 bands, ranging from 1.3 to 10<!--> <!-->Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7<!--> <!-->Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of <em>H. capsulatum</em> varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"45 3","pages":"Pages 423-428"},"PeriodicalIF":0.0000,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.015","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405001513","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12
Abstract
Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5–7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.
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