Oxidation of 14C-labeled substrates by hippocampal slice cultures

Abstract

In vitro studies of glucose and lactate utilization have been performed in acute hippocampal slices or dissociated neurons and glia. While some studies concluded that lactate and glucose are equivalent substrates to support evoked synaptic activity, others showed decreased synaptic activity in the presence of lactate as compared to glucose. We found diminished neural activity in the presence of lactate in hippocampal slice cultures. We developed a method to examine the oxidation rates of ¹⁴C-labeled substrates by hippocampal slice cultures. The rate of ¹⁴CO₂ production from either ¹⁴C-glucose or ¹⁴C-lactate remained unchanged for 6 h suggesting that slice cultures are metabolically stable. While the glucose oxidation rate saturated between 2.8 and 10 mM, lactate oxidation rate had not saturated at 10 mM. These data suggest that organotypic slice cultures provide a method to examine elements of cerebral metabolism in vitro.