Reciprocal Antagonism between MicroRNA-138 and SIRT1 and Its Implications for the Angiogenesis of Endothelial Cells.

IF 1.8 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Journal of Vascular Research Pub Date : 2021-02-03 DOI:10.1159/000511786
Shangyu Wang, Zengwu Shao, Xin Tang, Kaijie Wang, Jinping Zhao, Zhe Dong
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Abstract

MicroRNAs and sirtuins are important epigenetic regulators of gene expression and both contribute significantly to postnatal vascular development. However, the crosstalk between miRNAs and sirtuins in the modulation of angiogenesis has rarely been discussed. Here, we investigated the interactions between miR-138 and sirtuins in the process of angiogenesis. We found that overexpression of miR-138 markedly suppressed the proliferation, migration, and tube-forming capacities of the endothelial cells. And, miR-138 inhibitor-treated endothelial cells showed a reversed phenotype. Furthermore, miR-138 plays a negative role in vascular development in vivo. Western blot and qPCR assays demonstrated that SIRT1 was silenced by miR-138, and a luciferase reporter assay showed that miR-138 bound to the 3'-UTR of SIRT1. The re-expression of SIRT1 alleviated miR-138-mediated suppression of angiogenesis. Furthermore, silencing SIRT1 could boost the level of miR-138. And, upon miR-138 inhibitor treatment, SIRT1 silencing no longer reduced the angiogenic ability of endothelial cells significantly. These results demonstrated that the circuitry involving miR-138 and SIRT1 may participate in vascular homeostasis and also offered the possibility of identifying a new approach in the treatment of angiogenic diseases.

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MicroRNA-138 与 SIRT1 之间的相互拮抗作用及其对内皮细胞血管生成的影响
微RNA和sirtuins是基因表达的重要表观遗传调节因子,两者都对出生后的血管发育做出了重要贡献。然而,人们很少讨论 miRNA 与 sirtuins 在调节血管生成过程中的相互作用。在这里,我们研究了 miR-138 和 sirtuins 在血管生成过程中的相互作用。我们发现,过表达 miR-138 会明显抑制内皮细胞的增殖、迁移和管形成能力。而经 miR-138 抑制剂处理的内皮细胞则表现出逆转的表型。此外,miR-138 在体内血管发育中起着负面作用。Western 印迹和 qPCR 检测表明,SIRT1 被 miR-138 沉默,荧光素酶报告实验表明,miR-138 与 SIRT1 的 3'-UTR 结合。SIRT1 的重新表达缓解了 miR-138 介导的对血管生成的抑制。此外,沉默 SIRT1 可以提高 miR-138 的水平。而且,在接受 miR-138 抑制剂治疗后,沉默 SIRT1 不再能显著降低内皮细胞的血管生成能力。这些结果表明,涉及 miR-138 和 SIRT1 的回路可能参与了血管稳态,也为确定治疗血管生成疾病的新方法提供了可能性。
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来源期刊
Journal of Vascular Research
Journal of Vascular Research 医学-生理学
CiteScore
3.40
自引率
0.00%
发文量
25
审稿时长
>12 weeks
期刊介绍: The ''Journal of Vascular Research'' publishes original articles and reviews of scientific excellence in vascular and microvascular biology, physiology and pathophysiology. The scope of the journal covers a broad spectrum of vascular and lymphatic research, including vascular structure, vascular function, haemodynamics, mechanics, cell signalling, intercellular communication, growth and differentiation. JVR''s ''Vascular Update'' series regularly presents state-of-the-art reviews on hot topics in vascular biology. Manuscript processing times are, consistent with stringent review, kept as short as possible due to electronic submission. All articles are published online first, ensuring rapid publication. The ''Journal of Vascular Research'' is the official journal of the European Society for Microcirculation. A biennial prize is awarded to the authors of the best paper published in the journal over the previous two years, thus encouraging young scientists working in the exciting field of vascular biology to publish their findings.
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