Engineering Ustilago maydis for production of tailor-made mannosylerythritol lipids

IF 3.7 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Metabolic Engineering Communications Pub Date : 2021-06-01 DOI:10.1016/j.mec.2021.e00165
Fabienne Becker , Thorsten Stehlik , Uwe Linne , Michael Bölker , Johannes Freitag , Björn Sandrock
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引用次数: 14

Abstract

Mannosylerythritol lipids (MELs) are surface active glycolipids secreted by various fungi. MELs can be used as biosurfactants and are a biodegradable resource for the production of detergents or pharmaceuticals. Different fungal species synthesize a unique mixture of MELs differing in acetyl- and acyl-groups attached to the sugar moiety. Here, we report the construction of a toolbox for production of glycolipids with predictable fatty acid side chains in the basidiomycete Ustilago maydis. Genes coding for acyl-transferases involved in MEL production (Mac1 and Mac2) from different fungal species were combined to obtain altered MEL variants with distinct physical properties and altered antimicrobial activity. We also demonstrate that a U. maydis paralog of the acyltransferase Mac2 with a different substrate specificity can be employed for the biosynthesis of modified MEL variants. In summary, our data showcase how the fungal repertoire of Mac enzymes can be used to engineer tailor-made MELs according to specific biotechnological or pharmaceutical requirements.

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工程黑穗病菌用于生产量身定制的甘露糖赤藓糖醇脂
甘露糖赤四醇脂是多种真菌分泌的具有表面活性的糖脂。mel可以用作生物表面活性剂,是生产洗涤剂或药品的可生物降解资源。不同的真菌种类合成一种独特的mel混合物,这些mel的乙酰基和酰基与糖部分的连接不同。在这里,我们报告了一个工具箱的构建,用于生产糖脂具有可预测的脂肪酸侧链的担子菌黑穗病菌。研究人员将来自不同真菌物种的参与MEL生产的酰基转移酶(Mac1和Mac2)的编码基因组合在一起,获得了具有不同物理特性和抗菌活性的改变的MEL变体。我们还证明,具有不同底物特异性的U. maydis酰基转移酶Mac2类似物可用于修饰MEL变体的生物合成。总之,我们的数据展示了如何根据特定的生物技术或制药要求,利用Mac酶的真菌库来设计定制的mel。
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来源期刊
Metabolic Engineering Communications
Metabolic Engineering Communications Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
13.30
自引率
1.90%
发文量
22
审稿时长
18 weeks
期刊介绍: Metabolic Engineering Communications, a companion title to Metabolic Engineering (MBE), is devoted to publishing original research in the areas of metabolic engineering, synthetic biology, computational biology and systems biology for problems related to metabolism and the engineering of metabolism for the production of fuels, chemicals, and pharmaceuticals. The journal will carry articles on the design, construction, and analysis of biological systems ranging from pathway components to biological complexes and genomes (including genomic, analytical and bioinformatics methods) in suitable host cells to allow them to produce novel compounds of industrial and medical interest. Demonstrations of regulatory designs and synthetic circuits that alter the performance of biochemical pathways and cellular processes will also be presented. Metabolic Engineering Communications complements MBE by publishing articles that are either shorter than those published in the full journal, or which describe key elements of larger metabolic engineering efforts.
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