Antimicrobial Activity of Experimental Chitosan Solutions on Acrylic Resin and Cobalt-Chromium Surfaces.

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-11-01 DOI:10.11607/ijp.7264

Patrícia Almeida Curylofo, Priscilla Neves Raile, Viviane Cóssia de Oliveira, Ana Paula Macedo, Débora Fernandes Costa Guedes, Helena de Freitas Oliveira Paranhos, Valéria Oliveira Pagnano

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引用次数: 3

Abstract

Purpose: To evaluate the application of chitosan as a cleanser in the control of biofilm formation on cobalt-chromium (Co-Cr) alloy and acrylic resin surfaces.

Materials and methods: In total, 172 Co-Cr discs and 172 acrylic resin discs (14 mm x 3 mm) were contaminated with Streptococcus mutans, Staphylococcus aureus, Candida albicans, or Candida glabrata and incubated for 48 hours. Then, specimens were randomly divided into groups and immersed in the following solutions for 15 minutes: solution without chitosan (WC/control); chitosan solution (CH: 5 mg/mL); chitosan nanoparticle solu.on (CN: 3.8 mg/mL); and effervescent tablet (ET). Biofilm recovery rates (n = 9) were evaluated by counting the colony-forming units (CFU/mL). Biofilm morphology was evaluated using scanning electron microscopy (SEM). Data were compared using the Kruskal-Wallis or ANOVA tests followed by the Tukey post hoc test.

Results: For acrylic resin, ET showed the lowest number of CFU for S aureus and S mutans (P < .001). CH exhibited intermediate values for S mutans, S aureus, and C albicans; CN exhibited intermediate values for S mutans and S aureus. For C glabrata, there was no sta.s.cal difference between the solu.ons (P = .264). For Co-Cr, ET showed the highest level of antimicrobial action against all microorganisms (P < .001), and CH showed an intermediate level of action against S mutans and S aureus. Against C albicans and C glabrata, there was no significant difference among CH, CN, and WC.

Conclusions: Although ET had a broader spectrum of antimicrobial action, CH showed promise as a denture cleanser. Int J Prosthodont 2023;36:e61-e73.

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实验性壳聚糖溶液对丙烯酸树脂和钴铬表面的抗菌活性。

目的：评价壳聚糖作为清洁剂在控制钴铬合金和丙烯酸树脂表面生物膜形成方面的应用。材料和方法：总共172个Co-Cr椎间盘和172个丙烯酸树脂椎间盘（14mmx3mm）被变形链球菌、金黄色葡萄球菌、白色念珠菌或光滑念珠菌污染，并孵育48小时。然后，将样品随机分组，并在以下溶液中浸泡15分钟：不含壳聚糖的溶液（WC/对照）；壳聚糖溶液（CH:5mg/mL）；壳聚糖纳米粒子溶胶（CN:3.8mg/mL）；和泡腾片（ET）。通过计数菌落形成单位（CFU/mL）来评估生物膜回收率（n=9）。使用扫描电子显微镜（SEM）评估生物膜的形态。使用Kruskal-Wallis或ANOVA检验对数据进行比较，然后进行Tukey事后检验。结果：对于丙烯酸树脂，ET对金黄色葡萄球菌和变形链球菌的CFU数最低（P＜0.001）。CH对变形链球菌、金黄色葡萄杆菌和白色念珠菌的CFU值中等；CN对变异链球菌和金黄色葡萄球菌表现出中间值。对于玻璃藻，溶液之间没有sta.s.cal差异（P=.264）。对于Co-Cr，ET对所有微生物表现出最高水平的抗菌作用（P<.001），CH对变形链球菌和金黄色葡萄球菌表现出中等水平的抗菌活性。对于白色念珠菌和光滑念珠菌，CH、CN和WC之间没有显著差异。结论：尽管ET具有更广泛的抗菌作用，但CH显示出作为义齿清洁剂的前景。Int J Prostodont 2023；36:e61-e73。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.