{"title":"Eponymous plot of Richard J. Kitz and Irwin B. Wilson in biochemistry","authors":"Theodore A. Alston","doi":"10.1016/j.janh.2020.12.001","DOIUrl":null,"url":null,"abstract":"<div><p>Irwin B. Wilson and anesthesiologist Richard J. Kitz found the enzyme acetylcholinesterase to be inactivated in two steps by covalently acting molecules resembling acetylcholine in structure. Such molecules rapidly and reversibly bind to the active site of the enzyme. Next, the reversible complex undergoes covalent fixation at a characteristic rate. The Kitz-Wilson phenomenon applies to many cases of time-dependent enzyme inhibition. Experimental data are commonly graphed in linear fashion on “Kitz-Wilson plots”. Kitz also contributed to a <span>gas chromatography-mass spectrometry</span><svg><path></path></svg> assay for acetylcholine that was suitable for the nonbiological detection of that neurotransmitter in mammalian brain.</p></div>","PeriodicalId":38044,"journal":{"name":"Journal of Anesthesia History","volume":"6 4","pages":"Pages 3-4"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.janh.2020.12.001","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Anesthesia History","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S235245292030030X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Arts and Humanities","Score":null,"Total":0}
引用次数: 0
Abstract
Irwin B. Wilson and anesthesiologist Richard J. Kitz found the enzyme acetylcholinesterase to be inactivated in two steps by covalently acting molecules resembling acetylcholine in structure. Such molecules rapidly and reversibly bind to the active site of the enzyme. Next, the reversible complex undergoes covalent fixation at a characteristic rate. The Kitz-Wilson phenomenon applies to many cases of time-dependent enzyme inhibition. Experimental data are commonly graphed in linear fashion on “Kitz-Wilson plots”. Kitz also contributed to a gas chromatography-mass spectrometry assay for acetylcholine that was suitable for the nonbiological detection of that neurotransmitter in mammalian brain.
欧文·威尔逊(Irwin B. Wilson)和麻醉师理查德·基茨(Richard J. Kitz)发现，乙酰胆碱酯酶可以通过两个步骤被结构类似于乙酰胆碱的共价分子灭活。这样的分子能迅速且可逆地与酶的活性部位结合。然后，可逆复合物以特定速率进行共价固定。Kitz-Wilson现象适用于许多时间依赖性酶抑制的情况。实验数据通常在“基茨-威尔逊图”上以线性方式绘制。Kitz还为乙酰胆碱的气相色谱-质谱分析做出了贡献，该分析适用于哺乳动物大脑中神经递质的非生物检测。
期刊介绍：
The Journal of Anesthesia History (ISSN 2352-4529) is an international peer-reviewed journal dedicated to advancing the study of anesthesia history and related disciplines. The Journal addresses anesthesia history from antiquity to the present. Its wide scope includes the history of perioperative care, pain medicine, critical care medicine, physician and nurse practices of anesthesia, equipment, drugs, and prominent individuals. The Journal serves a diverse audience of physicians, nurses, dentists, clinicians, historians, educators, researchers and academicians.
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