The effect of caffeic acid phenethyl ester on cell cycle control gene expressions in breast cancer cells.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Research Communications Pub Date : 2021-03-01 DOI:10.22099/mbrc.2020.38811.1563

Tuğçe Balc-Okcanoğlu, Sunde Yilma-Susluer, Cagla Kayabasi, Besra Ozme-Yelken, Cigir Biray-Avci, Cumhur Gunduz

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引用次数: 6

Abstract

We aimed to find the effect of caffeic acid phenethyl ester (CAPE) on the expression profiles of cell cycle control genes in breast cancer cell line (MCF-7). The cytotoxic effect of CAPE on MCF-7 cell line was found with an XTT analysis. Total RNA was isolated from the cells exposed to IC₅₀ dose and untreated control cells. Expressions of genes related to cell cycle control (CCND2, RB1, ATM, CDC34, CDK5RAP1) were evaluated by qRT-PCR by the LightCycler 480 System (Roche). GAPDH and ACTB housekeeping genes were used for the normalization of gene expressions. IC₅₀ value of CAPE in MCF-7 cells was calculated as 75µM. It was shown that IC₅₀ dose of CAPE induced significant upregulation in expressions of cell cycle control genes, compared to control cells. CAPE increases the expression of genes that are important in cell cycle control, suggesting that this component can be used as an effective chemopreventive agent in breast cancer cells.

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咖啡酸苯乙酯对乳腺癌细胞周期调控基因表达的影响。

目的探讨咖啡酸苯乙酯(CAPE)对乳腺癌细胞系MCF-7细胞周期调控基因表达谱的影响。用XTT分析发现CAPE对MCF-7细胞株有细胞毒作用。从暴露于IC50剂量的细胞和未处理的对照细胞中分离总RNA。采用LightCycler 480系统(Roche)的qRT-PCR检测细胞周期控制相关基因(CCND2、RB1、ATM、CDC34、CDK5RAP1)的表达。GAPDH和ACTB管家基因用于基因表达的规范化。计算MCF-7细胞中CAPE的IC50值为75µM。结果表明，与对照细胞相比，IC50剂量的CAPE诱导细胞周期控制基因的表达显著上调。CAPE增加了在细胞周期控制中重要的基因的表达，这表明该成分可以作为乳腺癌细胞的有效化学预防剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biology Research Communications BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

0.00%

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期刊介绍： “Molecular Biology Research Communications” (MBRC) is an international journal of Molecular Biology. It is published quarterly by Shiraz University (Iran). The MBRC is a fully peer-reviewed journal. The journal welcomes submission of Original articles, Short communications, Invited review articles, and Letters to the Editor which meets the general criteria of significance and scientific excellence in all fields of “Molecular Biology”.