[Protective effects of polarizing cardioplegia with Na(+) channel inhibitor in ex vivo rat heart preservation].

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-10-01

Song-tao Tan, Shuang-qiang Yang, Chao-kun Yang

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Abstract

Objective: To investigate the protective effects of polarizing cardioplegia with Na(+) channel inhibitor tetrodotoxin (TTX) in ex vivo rat heart preservation.

Methods: Using a Langendorff preparation, Wistar rat hearts were arrested and preserved with St. Thomas (STH) solution (n=10) or with polarizing cardioplegia (TTX, n=10) for 7 h in hypothermic storage (10 degrees Celsius). All the hearts then underwent 30 min of reperfusion. Pre-ischemia and post-ischemia indexes of the rat hearts were determined, including the hemodynamic parameters, myocardial enzymology, ATP content and ultrastructural changes.

Results: The recovery of hemodynamic parameters of the hearts in TTX group were better than those in STH group (P<0.01). In comparison with STH group, the leakage of creatine kinase (CK) and lactate dehydrogenase (LDH) in TTX group was significantly lower (P<0.05) and ATP level maintained a relative high level with better protected myocardial ultrastructure.

Conclusion: TTX polarizing cardioplegia provides more effective long-term hypothermic preservation of isolated rat hearts than STH.

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[Na(+)通道抑制剂极化停搏对离体大鼠心脏保存的保护作用]。

目的:探讨Na(+)通道抑制剂河豚毒素(TTX)极化停搏对离体大鼠心脏的保护作用。方法:采用Langendorff制剂，取Wistar大鼠心脏，经St. Thomas (STH)溶液(n=10)或极化停搏液(TTX, n=10)低温保存7 h(10℃)。所有心脏进行30min再灌注。测定大鼠心脏缺血前、缺血后的血流动力学参数、心肌酶学、三磷酸腺苷(ATP)含量及超微结构变化。结果:TTX组心脏血流动力学参数恢复优于STH组(p)。结论:TTX极化心脏骤停对离体大鼠心脏的长期低温保存效果优于STH组。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA

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