[Sequence analysis of the mutated genes in CTXEVC Phi and nct-CTXnew Phi genomes in Vibrio cholerae JS94484 strain].

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Yong-yu Rui, Biao Kan, Shou-yi Gao, Yan-qing Liu, Guo-ming Qi

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Abstract

Objective: To analyze the sequences of the mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genomes in Vibrio cholerae JS94484 strain.

Methods: The mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genome were obtained by PCR, sequenced and analyzed.

Results: ig1, rstR, ig2, and ctxAB genes in CTX(EVC)Phi genome of V. cholerae strain JS94484 were highly homologous with those of standard EVC strain N16961, while ig1, rstR and ig2 genes in nct-CTX(new)Phi genome of the strain JS94484 shared low homology with those of the other 3 biotypes of V. cholerae. Considerable difference was detected in the last 60 bp of zot genes between CTX(EVC)Phi and nct-CTX(new)Phi genomes, indicating possible difference in the amino sequences of the Zot proteins encoded by these two genes. The sequence of toxin-coregulated pilus A subunit gene (tcpA) of the strain JS94484 was identical with that of strain N16961.

Conclusion: ig1, rstR and ig2 genes of nct-CTX(new)Phigenome are of a novel type, and their functions await further investigation.

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[霍乱弧菌JS94484株CTXEVC Phi和nct-CTXnew Phi基因组突变基因序列分析]。

目的:分析霍乱弧菌JS94484株CTX(EVC)Phi和nct-CTX(new)Phi基因组突变基因的序列。方法:采用PCR方法获得CTX(EVC)Phi和nct-CTX(new)Phi基因组突变基因，并对突变基因进行测序和分析。结果:JS94484霍乱弧菌CTX(EVC)Phi基因组ig1、rstR、ig2和ctxAB基因与EVC标准株N16961高度同源，而JS94484霍乱弧菌nct-CTX(新)Phi基因组ig1、rstR和ig2基因与其他3种生物型的同源性较低。CTX(EVC)Phi和nct-CTX(new)Phi基因组在zot基因的最后60 bp处存在显著差异，表明这两个基因编码的zot蛋白的氨基酸序列可能存在差异。菌株JS94484毒素协同调节菌毛A亚基基因(tcpA)序列与菌株N16961相同。结论:nct-CTX(new)表观基因组中ig1、rstR和ig2基因为新型基因，其功能有待进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA

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