[Oxidative stress-induced differentiation of L6 myoblasts].

Abstract

Objective: To explore the relationship between the differentiation of L6 myoblasts and oxidative stress.

Methods: MTT assay was used to determine the viability of L6 myoblasts, from which the total RNA was extracted for amplification of the myogenin gene fragment by RT-PCR. H(2)O(2)-induced morphological changes of the cells were observed.

Results: The myoblasts treated with low concentration of reactive oxygen (50 micromol/L H(2)O(2)) for 1 h exhibited accelerated cell growth (P<0.05), and treatment with 50 and 150 micromol/L H(2)O(2) induced the gene expression of myogenin, a molecular marker for differentiation of myoblasts. Morphological study revealed myotube formation and accelerated differentiation of the myoblasts induced by H(2)O(2).

Conclusion: The reactive oxygen may serve as the intracellular signal molecules to induce the growth and differentiation of the myoblasts.