[Expression of L-type calcium channel alpha1C subunit in adult rat heart].

Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-11-01

Yan Ou, Xiao-lin Niu, Fu-xian Ren, Ying Zhang, Feng-dong Ling

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Abstract

Objective: To investigate the expression and distribution of L-type calcium channel alpha1C subunits in adult rat heart.

Methods: HE staining was applied on the frozen sections of adult rat heart to identify the sinoatrial node (SAN), atrioventricular node (AVN), and posterior nodal extension (PNE). The protein expression of L-type calcium channel alpha1C in adult rat heart and its cellular localization were examined by Western blotting and immunohistochemistry, respectively.

Results: L-type calcium channel alpha1C subunit was immunolocalized on the membrane of the myocardial cells, and its expression increased gradually in the SAN, AVN, PNE, right atrium and right ventricle. The protein level of L-type calcium channel alpha1C in the AVN was similar to that in the PNE (P>0.05), and its level in the right atrium and ventricle were significantly higher than those in the SAN and AVN (P<0.01). The results of Western blotting were well consistent with the results of immunohistochemistry.

Conclusion: The expression of L-type calcium channel alpha1C subunit may play a role in the electrophysiological functions of the heart.

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成年大鼠心脏中l型钙通道α - 1c亚基的表达

目的:探讨成年大鼠心脏中l型钙通道α - 1c亚基的表达和分布。方法:采用HE染色对成年大鼠心脏冷冻切片进行窦房结(SAN)、房室结(AVN)和后结延伸(PNE)的鉴定。采用Western blotting和免疫组织化学方法分别检测成年大鼠心脏中l型钙通道α - 1c蛋白的表达及其细胞定位。结果:l型钙通道α 1c亚基免疫定位于心肌细胞膜上，其在SAN、AVN、PNE、右心房和右心室的表达逐渐升高。AVN中l型钙通道α 1c蛋白表达水平与PNE相似(P>0.05)，且其在右心房和心室的表达水平显著高于SAN和AVN (P)。结论:l型钙通道α 1c亚基的表达可能在心脏电生理功能中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA

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