Kelly M Johnson, Xavier Alvarez, Olga N Borkhsenious, H Michael Kubisch
{"title":"Nuclear and cytoplasmic localization of interferon-tau in in vitro-produced bovine blastocysts.","authors":"Kelly M Johnson, Xavier Alvarez, Olga N Borkhsenious, H Michael Kubisch","doi":"10.1051/rnd:2005060","DOIUrl":null,"url":null,"abstract":"<p><p>Experiments were conducted to detect interferon-tau in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN-tau in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN-tau in the trophectoderm of blastocysts. The distribution of IFN-tau appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.</p>","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"46 1","pages":"97-104"},"PeriodicalIF":0.0000,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005060","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction, nutrition, development","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1051/rnd:2005060","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
Abstract
Experiments were conducted to detect interferon-tau in bovine in vitro-derived blastocysts by transmission electron (TEM) and confocal microscopy. TEM showed the presence of IFN-tau in the cytoplasm and the nuclei of expanded blastocysts. Confocal microscopy similarly confirmed the presence of IFN-tau in the trophectoderm of blastocysts. The distribution of IFN-tau appeared variable with some cells showing strong labeling while others appeared to be devoid of the protein.
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