In vitro three-stage continuous fermentation of gluco-oligosaccharides produced by Gluconobacter oxydans NCIMB 4943 by the human colonic microflora.

S Wichienchot, P Prasertsan, T Hongpattarakere, G R Gibson, R A Rastall
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Abstract

Gluco-oligosaccharides produced by Gluconobacter oxydans NCIMB 4943 from maltodextrin as the source, were evaluated for their fermentability by the human colonic microflora. The selectivity of growth of desirable bacteria in the human colon was studied in a three-stage continuous model of the human large intestine. Populations of bacteria, and their fluctuations as a response to the fermentation, were enumerated using fluorescent in situ hybridization (FISH). The gluco-oligosaccharides resulted in increases in numbers of bifidobacteria and the Lactobacillus/Enterococcus group in all 3 vessels of the system, representing the proximal, transverse and distal colonic areas. The prebiotic indices of the gluco-oligosaccharides were 2.29, 4.23 and 2.74 in V1, V2 and V3 respectively.

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氧化葡萄糖杆菌NCIMB 4943产生的低聚葡萄糖在体外三段式连续发酵的研究。
以麦芽糖糊精为原料,利用人结肠菌群对氧化葡萄糖杆菌NCIMB 4943生产的低聚糖进行了发酵性评价。在人大肠三阶段连续模型中,研究了理想细菌在人结肠中的生长选择性。利用荧光原位杂交(FISH)对细菌种群及其波动进行了枚举。低聚糖导致双歧杆菌和乳杆菌/肠球菌群数量增加,在系统的所有3个血管中,代表近端、横向和远端结肠区域。低聚糖在V1、V2和V3期的益生元指数分别为2.29、4.23和2.74。
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