Rac2 concentrations in umbilical cord neutrophils.

Biology of the neonate Pub Date : 2006-01-01 Epub Date: 2006-03-30 DOI:10.1159/000092451

Virginia M Meade, Cecilia N Barese, Chaekyun Kim, Charles G Njinimbam, Christophe C Marchal, David A Ingram, D Wade Clapp, Mary C Dinauer, Mervin C Yoder

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引用次数: 11

Abstract

Background: Human newborn infants display a variety of immunodeficiencies of immaturity, including diminished neutrophil adhesion, chemotaxis, and migration. Rac2, a guanosine triphosphate-binding protein, is an essential regulator of human neutrophil migration and chemotaxis. Since human subjects and mice deficient in Rac2 display deficiencies in neutrophil functions similar to newborn infants, we postulated that newborn neutrophils may be deficient in Rac2.

Objectives: The aim of the study was to measure Rac1 and Rac2 concentrations in neutrophils from umbilical cord blood.

Methods: Neutrophils from cord and adult blood were isolated, total cell lysates extracted, and Rac protein concentrations determined using Western blot analysis.

Results: Rac2 concentrations were significantly lower in the neutrophil protein lysates isolated from cord blood compared to adult blood despite similar levels of Rac1.

Conclusions: Diminished Rac2 expression in cord blood neutrophils may contribute to the defects observed in cord blood neutrophil function.

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脐带中性粒细胞中的 Rac2 浓度。

背景：人类新生儿表现出各种不成熟的免疫缺陷，包括中性粒细胞粘附性、趋化性和迁移性减弱。Rac2 是一种三磷酸鸟苷结合蛋白，是人类中性粒细胞迁移和趋化的重要调节因子。由于缺乏 Rac2 的人体和小鼠在中性粒细胞功能方面表现出与新生儿相似的缺陷，我们推测新生中性粒细胞可能缺乏 Rac2：研究旨在测量脐带血中性粒细胞中 Rac1 和 Rac2 的浓度：方法：分离脐带血和成人血中的中性粒细胞，提取总细胞裂解液，使用Western印迹分析测定Rac蛋白浓度：结果：与成人血液相比，从脐带血中分离出的中性粒细胞蛋白裂解液中的Rac2浓度明显较低，尽管Rac1的含量相似：结论：脐带血中性粒细胞中 Rac2 表达减少可能是导致脐带血中性粒细胞功能缺陷的原因之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biology of the neonate

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