Yong-Hyun Shin , Guy Wilhem Lee , Kyung-No Son , Sang Min Lee , Chang Joong Kang , Byoung S. Kwon , Jiyoung Kim
{"title":"Promoter analysis of human CC chemokine CCL23 gene in U937 monocytoid cells","authors":"Yong-Hyun Shin , Guy Wilhem Lee , Kyung-No Son , Sang Min Lee , Chang Joong Kang , Byoung S. Kwon , Jiyoung Kim","doi":"10.1016/j.bbaexp.2007.01.012","DOIUrl":null,"url":null,"abstract":"<div><p>Expression of CCL23 is induced by external stimuli including PMA in monocytes, but its transcriptional regulation has not been studied to date. Serial deletion analysis of its 5′ flanking region revealed that the region −<!--> <!-->293 to +<!--> <!-->31 was important for induction by PMA. <em>Cis</em>-acting elements at the −<!--> <!-->269/−<!--> <!-->264 (NFAT site), −<!--> <!-->167/−<!--> <!-->159 (NF-κB site), and −<!--> <!-->51/−<!--> <!-->43 (AP-1 site) positions were identified as the critical sites for the CCL23 expression in U937 cells. We demonstrated the binding of the transcription factors to the consensus sites. Specific inhibitors for signal pathways reduced PMA-induced expression of CCL23, confirming involvement of these transcription factors.</p></div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1769 3","pages":"Pages 204-208"},"PeriodicalIF":0.0000,"publicationDate":"2007-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2007.01.012","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167478107000334","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
Expression of CCL23 is induced by external stimuli including PMA in monocytes, but its transcriptional regulation has not been studied to date. Serial deletion analysis of its 5′ flanking region revealed that the region − 293 to + 31 was important for induction by PMA. Cis-acting elements at the − 269/− 264 (NFAT site), − 167/− 159 (NF-κB site), and − 51/− 43 (AP-1 site) positions were identified as the critical sites for the CCL23 expression in U937 cells. We demonstrated the binding of the transcription factors to the consensus sites. Specific inhibitors for signal pathways reduced PMA-induced expression of CCL23, confirming involvement of these transcription factors.
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