mED2—A Novel Gene Involved in Mouse Embryonic Development

DUAN Jia-Zhong , ZHANG Jing-Pu , ZHU Shao-Xia
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引用次数: 3

Abstract

Dissection of new genes underlying embryonic development is important for our understanding of the molecular mechanism of vertebrate embryonic development. In this study, the expression pattern and functional analysis of a new gene, called mED2, originally cloned from mouse embryos using subtractive hybridization was reported. mED2 expression patterns were characterized by RT-PCR-Southern hybridization and in situ hybridization. The results showed that mED2 was mainly expressed in the embryonic nervous system and mesoderm-derived tissues and its expression varied depending on the embryonic developmental stages. The knockdown of mED2 activity by antisense RNA injection inhibited zygote cleavage and blastocyst formation during pre-implantation in mice. Subcellular localization of mED2-eGFP fusion protein revealed a pattern of nuclear membrane and juxta-/perinuclear location such as in the rough endoplasmic reticulum and Golgi apparatus. This finding was supported by bioinformatics analysis, which indicated mED2 protein to be a transmembrane protein with partial homology to the thioredoxin family of proteins. It is inferred that mED2 gene can probably take part in early embryonic development in mouse and may be involved in target protein posttranslational modification, turnover, folding, and stability at the endoplasmic reticulum and/or the Golgi apparatus.

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mED2-A参与小鼠胚胎发育的新基因
解剖胚胎发育的新基因对我们理解脊椎动物胚胎发育的分子机制非常重要。在这项研究中,报道了一个新的基因,称为mED2的表达模式和功能分析,最初是用减法杂交从小鼠胚胎中克隆出来的。通过RT-PCR-Southern杂交和原位杂交对mED2的表达模式进行了表征。结果表明,mED2主要表达于胚胎神经系统和中胚层组织中,其表达随胚胎发育阶段的不同而不同。注射反义RNA抑制mED2活性抑制小鼠着床前受精卵裂解和囊胚形成。mED2-eGFP融合蛋白的亚细胞定位揭示了核膜和核旁/核周定位的模式,如粗内质网和高尔基体。这一发现得到了生物信息学分析的支持,表明mED2蛋白是一个跨膜蛋白,与硫氧还蛋白家族部分同源。推测mED2基因可能参与小鼠早期胚胎发育,并可能参与靶蛋白在内质网和/或高尔基体的翻译后修饰、翻转、折叠和稳定性。
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