The ins and outs of ATP-dependent chromatin remodeling in budding yeast: Biophysical and proteomic perspectives

Joke J.F.A. van Vugt , Michael Ranes , Coen Campsteijn , Colin Logie
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引用次数: 53

Abstract

ATP-dependent chromatin remodeling is performed by multi-subunit protein complexes. Over the last years, the identity of these factors has been unveiled in yeast and many parallels have been drawn with animal and plant systems, indicating that sophisticated chromatin transactions evolved prior to their divergence. Here we review current knowledge pertaining to the molecular mode of action of ATP-dependent chromatin remodeling, from single molecule studies to genome-wide genetic and proteomic studies. We focus on the budding yeast versions of SWI/SNF, RSC, DDM1, ISWI, CHD1, INO80 and SWR1.

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芽殖酵母中atp依赖性染色质重塑的来龙外脉:生物物理和蛋白质组学观点
atp依赖性染色质重塑是由多亚基蛋白复合物完成的。在过去的几年里,这些因子的身份已经在酵母中被揭开,并且与动物和植物系统有许多相似之处,表明复杂的染色质交易在它们的分化之前就已经进化了。在这里,我们回顾了目前有关atp依赖性染色质重塑的分子作用模式的知识,从单分子研究到全基因组遗传和蛋白质组学研究。我们重点研究了SWI/SNF、RSC、DDM1、ISWI、CHD1、INO80和SWR1的出芽酵母版本。
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