The Correlation of Reproduction-Related Gene Expression with Germ Cell Number in DM and PLL Gilts

HU Yan-Biao , PAN Zeng-Xiang , XU Dan , XU Yin-Xue , LIU Hong-Lin , HUANG Rui-Hua , HU Zhi-Gang
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引用次数: 4

Abstract

In this study, the ovarian germ cell number was counted in 3-week-old Duroc × Meishan (DM, n=30) and PIC × (Landrace × Large White) (PLL, n=53) gilts, and the mRNA expression levels of four reproduction-related genes were investigated by quantitative RT-PCR. Correlation of germ cell number with the expression level of these genes was analyzed. Results showed that the germ cell number of DM was significantly higher than that of PLL gilts (P<0.01), although there was no significant difference between the ovarian weight of DM and PLL gilts (P=0.269). No significant correlation existed between germ cell number and ovarian weight in the two gilt groups (R=0.335, P=0.07; R=0.119, P=0.398, respectively). A significant correlation was found between the germ cell number and expression level of ESR and IGF1R mRNA in DM gilts (R=0.648, P<0.05; R=0.757, P<0.01, respectively), but the correlation between the germ cell number and expression level of FSHR and INHBA mRNA did not reach statistical significance. Significant correlation was found between the germ cell number and the expression level of ESR, FSHR, and IGF1R mRNA in PLL gilts (R=0.435, P<0.01; R=0.438, P<0.01; R=0.292, P<0.05, respectively), but not with INHBA mRNA in PLL gilts.

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DM和PLL后备母猪生殖相关基因表达与生殖细胞数量的关系
本研究以3周龄“杜×梅山”(DM, n=30)和“大×长×大白”(PLL, n=53)后备母猪为实验材料,计数卵巢生殖细胞数量,并采用定量RT-PCR方法检测4种生殖相关基因的mRNA表达水平。分析了这些基因的表达水平与生殖细胞数量的关系。结果表明:DM母猪的生殖细胞数量极显著高于PLL母猪(P<0.01),但DM母猪卵巢重量与PLL母猪无显著差异(P=0.269)。两组母猪生殖细胞数与卵巢重量无显著相关(R=0.335, P=0.07;R=0.119, P=0.398)。DM后备母猪生殖细胞数量与ESR和IGF1R mRNA表达水平呈显著相关(R=0.648, p < 0.05;R=0.757, P<0.01),但生殖细胞数量与FSHR和INHBA mRNA表达水平的相关性无统计学意义。PLL后备母猪生殖细胞数与ESR、FSHR、IGF1R mRNA表达量呈极显著相关(R=0.435, P<0.01;R = 0.438,术中;0.01;R=0.292, P<0.05),但在PLL后备母猪中与INHBA mRNA无关。
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