Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León.

J A Salinas-Mélendez, S Galván de la Garza, V M Riojas-Valdés, A Wong González, R Avalos-Ramírez
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Abstract

The aim of the present study was to determine the presence of Borrelia burgdorferi antibodies in horses from the metropolitan area of Monterrey, Nuevo León, México. Blood serum was obtained from a total of 100 horses residing at different counties in the area. From each animal data was obtained on age, sex, county of residence, presence of ectoparasites and clinical signs. All sera samples were analyzed by indirect immunofluoresence and the sera that resulted positive to this test was analyzed by Western blot. The serological test yielded 34 positive sera at 1:64 dilution, and from them 6 were positive at 1:128 dilution, 3 at 1:256, and only one at 1:512. Confirmation of the infection by Western blot was obtained only in the sample positive at the 1:512 dilution. These results shown a low frequency of seropositivity to B. burgdorferi of the horses in the area, confirming previous studies indicating that in northeast Mexico Lyme disease is present in different animal species.

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新墨西哥州蒙特雷郊区马的伯氏疏螺旋体抗体检测León。
本研究的目的是确定在蒙特雷大都市区、新墨西哥州León、墨西哥马体内是否存在伯氏疏螺旋体抗体。采集了居住在该地区不同县的100匹马的血清。从每只动物中获得年龄、性别、居住县、有无体外寄生虫和临床症状的数据。所有血清样本均采用间接免疫荧光法进行分析,阳性血清采用Western blot法进行分析。以1:64稀释得到34份阳性血清,以1:8 8稀释得到6份阳性血清,以1:6 6 256稀释得到3份阳性血清,以1:512稀释得到1份阳性血清。只有在1:512稀释后呈阳性的样品中,Western blot才能证实感染。这些结果表明,该地区马对伯氏疏螺旋体血清呈阳性的频率较低,证实了先前的研究表明,在墨西哥东北部,莱姆病存在于不同的动物物种中。
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