Analysis of Amylose Accumulation During Seed Development in Maize

GUO Shang-Jing , LI Jia-Rui , QIAO Wei-Hua , ZHANG Xian-Sheng
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引用次数: 13

Abstract

Starch, which includes amylose and amylopectin, is the most important component in maize (Zea mays L.) seeds. The accumulation of amylose in maize seeds was examined in this study. The percentage of amylose content gradually increased in seeds from day 10 to day 25 after pollination, which is consistent with the changes of GBSS activity. The transcripts of GBSSI were detected in both the endosperm and embryo of wild-type maize. However, its transcripts, GBSS activity, and amylose were not detected in either the endosperm or embryo of waxy maize. These results indicate that the accumulation of amylose is controlled by GBSSI expression in the seeds of maize.

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玉米种子发育过程中直链淀粉积累的分析
淀粉是玉米(Zea mays L.)种子中最重要的成分,包括直链淀粉和支链淀粉。对玉米种子中直链淀粉的积累进行了研究。授粉后第10 ~ 25天,种子直链淀粉含量百分比逐渐升高,这与GBSS活性的变化相一致。在野生型玉米胚乳和胚中均检测到GBSSI转录本。然而,在糯玉米胚乳和胚中均未检测到其转录本、GBSS活性和直链淀粉。说明玉米种子中直链淀粉的积累受GBSSI表达的控制。
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