Characterization of the Drosophila insulin receptor promoter

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-04-01 DOI:10.1016/j.bbaexp.2007.03.003

Sergio Casas-Tinto , Michael T. Marr II , Pedro Andreu , Oscar Puig

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引用次数: 15

Abstract

The insulin receptor (InR) signaling pathway is largely conserved in metazoans and it is required for normal growth and development in Drosophila. Despite the importance of this pathway in regulating growth, development and metabolism in Drosophila, little is known about how dInR expression is controlled in flies. Here we report the characterization of the dInR gene promoter and the analysis of its expression during embryo development. Drosophila InR gene has three promoters spanning 40 kb in the genome. These promoters direct the expression of three distinct mRNA transcripts that share common exons downstream of the initiator codon ATG but have different 5′UTRs. All three promoters are differentially regulated, spatially and temporally, contributing to a very complex pattern of expression in the developing embryo. Our results indicate that dInR expression in Drosophila displays an intricate pattern of regulation that assures an adequate control of growth, development and metabolism.

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果蝇胰岛素受体启动子的表征

胰岛素受体(InR)信号通路在后生动物中很大程度上是保守的，它是果蝇正常生长发育所必需的。尽管这一途径在调节果蝇生长、发育和代谢方面具有重要意义，但对于果蝇中如何控制dInR表达却知之甚少。在这里，我们报道了dInR基因启动子的特征及其在胚胎发育过程中的表达分析。果蝇InR基因在基因组中有3个启动子，全长40kb。这些启动子指导三种不同mRNA转录物的表达，这些转录物在启动子ATG下游具有共同的外显子，但具有不同的5 ' utr。这三种启动子在空间和时间上都受到不同的调控，在发育中的胚胎中形成了非常复杂的表达模式。我们的研究结果表明，果蝇的dInR表达显示出一种复杂的调节模式，以确保对生长、发育和代谢的充分控制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

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