Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2007-07-01 DOI:10.1016/j.bbaexp.2007.04.006

Tetsuya Chujo , Ryota Takai , Chiharu Akimoto-Tomiyama , Sugihiro Ando , Eiichi Minami , Yoshiaki Nagamura , Hanae Kaku , Naoto Shibuya , Michiko Yasuda , Hideo Nakashita , Kenji Umemura , Atsushi Okada , Kazunori Okada , Hideaki Nojiri , Hisakazu Yamane

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引用次数: 146

Abstract

We present a detailed characterization of the chitin oligosaccharide elicitor-induced gene OsWRKY53. OsWRKY53 was also induced in suspension-cultured rice cells by a fungal cerebroside elicitor and in rice plants by infection with the blast fungus Magnaporthe grisea. A fusion of OsWRKY53 with green fluorescent protein was detected exclusively in the nuclei of onion epidermal cells, and OsWRKY53 protein specifically bound to W-box elements. A transient assay using the particle bombardment method showed that OsWRKY53 is a transcriptional activator. A microarray analysis revealed that several defense-related genes, including pathogenesis-related protein genes such as PBZ1, were upregulated in rice cells overexpressing OsWRKY53. Finally, overexpression of OsWRKY53 in rice plants resulted in enhanced resistance to M. grisea. These results strongly suggest that OsWRKY53 is a transcription factor that plays important roles in elicitor-induced defense signaling pathways in rice.

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激发子诱导基因OsWRKY53参与水稻防御相关基因的表达

我们提出了几丁质寡糖激发子诱导基因OsWRKY53的详细表征。OsWRKY53在悬浮培养的水稻细胞和稻瘟病菌Magnaporthe grisea侵染的水稻植株中也有诱导作用。在洋葱表皮细胞的细胞核中检测到OsWRKY53与绿色荧光蛋白的融合，OsWRKY53蛋白与W-box元件特异性结合。用粒子轰击法进行的瞬时实验表明，OsWRKY53是一种转录激活剂。微阵列分析显示，在过表达OsWRKY53的水稻细胞中，包括致病相关蛋白基因(如PBZ1)在内的几种防御相关基因上调。最后，OsWRKY53在水稻中的过表达导致水稻对稻瘟病菌的抗性增强。这些结果强烈表明OsWRKY53是一个在水稻激发剂诱导的防御信号通路中起重要作用的转录因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

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