Treatment of scrapie pathogen 263K with tetracycline partially abolishes protease-resistant activity in vitro and reduces infectivity in vivo.

Biomedical and environmental sciences : BES Pub Date : 2007-06-01

Yan-Jun Guo, Jun Han, Hai-Lan Yao, Bao-Yun Zhang, Jian-Mei Gao, Jin Zhang, Xin-Li Xiao, Xiao-Fan Wang, Wei-Qin Zhao, De-Xin Wang, Xiao-Ping Dong

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Abstract

Objective: To study the possible effect of tetracycline on protease-resistant activity in vitro and infectivity in vivo of a scrapie strain 263K.

Methods: Scrapie pathogens were incubated with tetracycline at different concentrations for various periods of time and protease-resistant PrP signals were evaluated with proteinase K-treatment and Western blots. The preparations treated with tetracycline were intracerebrally inoculated into golden hamsters and typical TSE manifestations were noted. PrPSc in brain tissues of the infected animals was detected by PrP specific Western blot assays.

Results: Protease-resistant PrP was significantly reduced in or removed from the preparations treated with tetracycline in a dose-dependant manner. Compared with the control group after incubated for 53.75 +/- 0.50 days, the preparations treated with 5 mmol/L and 20 mmol/L tetracycline prolonged the incubation time of 61.5 +/- 1.73 and 59.5 +/- 0.58 days (P < 0.05).

Conclusion: Treatment of scrapie pathogen 263K with tetracycline reduces or removes its protease-resistant activity in vitro.

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用四环素治疗痒病病原体263K可部分消除体外蛋白酶抗性活性并降低体内感染性。

目的:研究四环素对痒病菌株263K体外蛋白酶耐药活性和体内感染性的可能影响。方法:用不同浓度的四环素孵育痒病病原菌不同时间，用蛋白酶k处理和Western blots检测耐蛋白酶PrP信号。用四环素处理的制剂脑内接种金仓鼠，观察到典型的TSE表现。采用PrP特异性Western blot检测感染动物脑组织中的PrPSc。结果:在四环素处理的制剂中，蛋白酶抗性PrP显著减少或去除，并呈剂量依赖性。与对照组相比，5 mmol/L和20 mmol/L四环素组孵育时间分别延长了61.5 +/- 1.73和59.5 +/- 0.58 d (P < 0.05)。结论:四环素治疗瘙痒病病原菌263K可降低或消除其蛋白酶耐药活性。

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