[Exploring genetic diversity in Dioscorea zingiberensis by amplified fragment length polymorphism molecular markers].

Abstract

Amplified fragment length polymorphism (AFLP) was used to study 30 individuals from 5 wild populations of Dioscorea zingiberensis for the first time. A total of 14 698 bands were detected with 9 pairs of AFLP primers and 12 686 of them were polymorphic. On the average each primer combination could be used to detect 230 polymorphic bands and account for 85.92% of total genetic diversity at species level. Shannonos index of diversity (I) was 0.3656-/+0.1721, and Nei's gene diversity (H) was 0.2322-/+0.2200 at the species level. The result of genetic variance analysis showed the coefficient of genetic differentiation (Gst) was 0.4827 at species level, it indicated there were certain degree of genetic differentiation in five Dioscorea zingiberensis populations. The gene flow (Nm) among populations of D. zingiberensis was 0.5358. The data were analyzed using unweighted pair group method, basing on arithmetic averages (UPGMA) bootstrap analysis. Cluster analyses were performed by using NTSYSpc version 2.11F and Popgene 1.32 software. The results showed that the genetic differentiation of 5 wild populations of D. zingiberensis was abundant, and 5 wild populations of D. zingiberensis could be clustered by the distance of the position basically. The AFLP molecular marker was used to identify the genetic differences of different populations of Dioscorea zingiberensis.