A novel role of classical swine fever virus E(rns) glycoprotein in counteracting the newcastle disease virus (NDV)-mediated IFN-beta Induction.

Journal of biochemistry and molecular biology Pub Date : 2007-09-30 DOI:10.5483/bmbrep.2007.40.5.611

Yan-hua Xia, Liu Chen, Zi-shu Pan, Chu-yu Zhang

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引用次数: 15

Abstract

E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) and has an unusual feature of RNase activity. In the present study, we demonstrate that E(rns) counteracts Newcastle disease virus (NDV)-mediated induction of IFN-beta. For this purpose, E(rns) fused to the enhanced green fluorescent protein (EGFP) was transiently expressed in porcine kidney 15 (PK15) cells. In luciferase activity assay, E(rns)-EGFP was found to prevent IFN-beta promoter-driven luciferase expression and block the induction of IFN-beta promoter mediated by NDV in a dosedependent manner. Through IFN-specific semi-quantitative RT-PCR detection, obvious decrease of IFN-beta mRNA in NDV-infected PK15 cells was observed in the presence of E(rns)-EGFP. In contrast, EGFP alone showed none of this block capacity. In addition, E(rns)-EGFP mutations with RNase inactivation were also found to block NDV-mediated induction of IFN-beta. These evidences establish a novel function for CSFV E(rns) glycoprotein in counteraction of the IFN-beta induction pathway.

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经典猪瘟病毒E(rns)糖蛋白在对抗新城疫病毒(NDV)介导的ifn - β诱导中的新作用

E(rns)是猪瘟病毒(CSFV)的一种包膜糖蛋白，具有RNase活性。在本研究中，我们证明了E(rns)可以抵消新城疫病毒(NDV)介导的ifn - β的诱导。为此，E(rns)与增强的绿色荧光蛋白(EGFP)融合，在猪肾15 (PK15)细胞中短暂表达。在荧光素酶活性测定中，我们发现E(rns)-EGFP可以抑制ifn - β启动子驱动的荧光素酶表达，并以剂量依赖的方式阻断NDV介导的ifn - β启动子的诱导。通过ifn特异性半定量RT-PCR检测，在E(rns)-EGFP存在下，ndv感染的PK15细胞中ifn - β mRNA明显降低。相比之下，单独EGFP没有表现出这种阻滞能力。此外，RNase失活的E(rns)-EGFP突变也被发现可以阻断ndv介导的ifn - β的诱导。这些证据建立了猪瘟病毒糖蛋白在对抗ifn - β诱导途径中的新功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of biochemistry and molecular biology

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