IGF2/Mboll polymorphism in Gir and Nelore cattle.

Abstract

The imprinted gene insulin-like growth factor 2 (IGF2) carries out important functions in the development of placental mammals, during the embryonic and fetal stages and is located on bovine chromosome 29. The main aim of this study was to verify the occurrence of a polymorphism (C/T transversion) in exon 6 of the IGF2 gene in Gir and Nelore cattle, Zebu breeds of great economic importance in Brazil for dairy and beef production, respectively. A 193 bp fragment of bovine IGF2 exon 6 was amplified by PCR with specific primers for this region and digested with Mboll enzyme to analyze the polymorphic segment. In a total of 39 Gir animals (29 females and 10 males), the estimated frequencies of the C and T alleles were 0.42 and 0.58, respectively. The genotypic frequencies were 0.10 for CC, 0.26 for TT and 0.64 for CT. For 28 Nelore animals (17 females and 11 males), the estimated frequencies of the C and T alleles were 0.43 and 0.57, respectively. The genotypic frequencies were 0.18 for CC, 0.32 for TT, and 0.50 for CT. This is the first report of the occurrence of this polymorphism in these breeds. The polymorphic site can be a regulatory motif with functional significance to gene regulation of the IGF2 gene. This polymorphism could be used to investigate the allele-specific expression of the IGF2 gene, its epigenetic status, and its role in developmental, growth and reproductive traits.