Development and analytical performance of an automated screening method for cannabinoids on the Dimension clinical chemistry system.

The Journal of Automatic Chemistry Pub Date : 1997-01-01 DOI:10.1155/S1463924697000187

D M Obzansky, E G Gorman, S P Kramer, I S Masulli, E A Nuzzaci, W F Skogen

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引用次数: 1

Abstract

A fully automated, random access method for the determination of cannabinoids (UTHC) was developed for the Dimension AR and XL clinical chemistry systems. The method utilizes Abuscreen ONLINE reagents and a multianalyte liquid calibrator containing 11-nor-Delta(9)-THC-9-carboxylic acid. Within-run and total reproducibility, determined using NCCLS protocol EP5- T2, was less than 0.6% and 1.6% CV, respectively, at all concentrations. Calibration stability was retained for at least 30 days. An extensive evaluation of non-structurally related drugs and various physiological substances indicated lack of interference in the method. No sample carry-over was observed following a specimen containing 1886 ng/ml 11-nor-Delta(9)-THC-9-carboxylic acid. A 99.1% agreement (N = 445 samples) was found between an EMIT based method on the aca discrete clinical analyser and the Dimension UTHC method.Dimension clinical chemistry system and aca discrete clinical analyser are registered trademarks of Dade International.

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维格临床化学系统大麻素自动筛选方法的建立及分析性能。

为Dimension AR和XL临床化学系统开发了一种全自动，随机存取的大麻素(UTHC)测定方法。该方法使用Abuscreen ONLINE试剂和含有11-no - delta (9)- thc -9-羧酸的多分析液校准器。在所有浓度下，使用NCCLS方案EP5- T2测定的运行内重复性和总重复性分别小于0.6%和1.6% CV。校准稳定性保持至少30天。对非结构相关药物和各种生理物质的广泛评估表明，该方法缺乏干扰。在含有1886 ng/ml 11- No - delta (9)- thc -9-羧酸的样品后，未观察到样品携带。在aca离散临床分析仪上基于EMIT的方法与Dimension UTHC方法之间发现99.1%的一致性(N = 445个样本)。Dimension临床化学系统和aca离散临床分析仪是戴德国际的注册商标。

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