Investigations into the interaction thermodynamics of TRAP-related peptides with a temperature-responsive polymer-bonded porous silica stationary phase

IF 2.5 Q1 Chemistry Analytica Chimica Acta: X Pub Date : 2019-03-01 DOI:10.1016/j.acax.2019.100008
Jamil Chowdhury, Reinhard I. Boysen, Milton T.W. Hearn
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Abstract

The interaction thermodynamics of the thrombin receptor agonistic peptide (TRAP-1), H-Ser-Phe-Leu-Leu-Arg-Asn-Pro-OH, and a set of alanine scan substitution peptides, have been investigated with an n-octadecylacrylic polymer-bonded porous silica (Sil-ODA18) and water-acetonitrile mobile phases at temperatures ranging from 5 to 80 °C in 5 °C increments. The retention of these peptides on the Sil-ODA18 stationary phase decreased as the water content in the mobile phase was lowered from 80% (v/v) to ca. 45% (v/v) and reached a minimum value for each peptide at a specific water-acetonitrile composition. Further decreases in the water content of the mobile phase led to increased retention. The magnitude of the changes in enthalpy of interaction, ΔHassoc0, changes in entropy of interaction, ΔSassoc0, and changes in heat capacity, ΔCp0, were found to be dependent on the molecular properties of the mobile phase, the temperature, the structure/mobility of the stationary phase, and the conformation and solvation state of the peptides. With water-rich mobile phases, the retention behaviour of the TRAP analogues was dominated by enthalpic processes, consistent with the participation of strong hydrogen bonding effects, but became dominated by entropic effects with acetonitrile-rich mobile phases as the temperature was increased. These changes in the retention behaviour of these TRAP peptides are consistent with the generation of water or acetonitrile clusters in the mobile phase depending on the volume fractions of the organic solvent as the Sil-ODA18 stationary phase transitions from its crystalline to its isotropic state.

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温度响应型聚合物键合多孔二氧化硅固定相与trap相关肽相互作用热力学的研究
研究了凝血酶受体激动肽(trap1), h - ser - ph - leu - leu - arg - asn - pro - oh和一组丙氨酸扫描取代肽,在n-十八烷基丙烯酸聚合物键合多孔二氧化硅(Sil-ODA18)和水-乙腈流动相下,在5至80 °C的温度范围内,以5 °C的速度增加的相互作用热力学。随着流动相中含水量从80% (v/v)降低到45% (v/v),这些肽在Sil-ODA18固定相上的保留率降低,并且在特定的水-乙腈组成下达到最小值。流动相含水量的进一步降低导致保留率的增加。相互作用焓变化的大小ΔHassoc0、相互作用熵变化的大小ΔSassoc0和热容变化的大小ΔCp0取决于流动相的分子性质、温度、固定相的结构/迁移率以及多肽的构象和溶剂化状态。在富水流动相中,TRAP类似物的保留行为以焓过程为主,与强氢键效应的参与一致;而在富乙腈流动相中,随着温度的升高,TRAP类似物的保留行为以熵作用为主。当Sil-ODA18固定相从晶体状态转变为各向同性状态时,这些TRAP肽保留行为的变化与流动相中水或乙腈团簇的产生是一致的,这取决于有机溶剂的体积分数。
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来源期刊
Analytica Chimica Acta: X
Analytica Chimica Acta: X Chemistry-Analytical Chemistry
自引率
0.00%
发文量
3
审稿时长
16 weeks
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