Protease-activated receptor 2 mediates interleukin-8 and intercellular adhesion molecule-1 expression in response to Aggregatibacter actinomycetemcomitans.

Oral microbiology and immunology Pub Date : 2009-08-01 DOI:10.1111/j.1399-302X.2009.00507.x

T Shimada, N Sugano, K Ikeda, K Shimada, T Iizuka, K Ito

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引用次数: 13

Abstract

Introduction: We investigated the mechanisms by which extracts of Aggregatibacter actinomycetemcomitans affect the inflammatory response in gingival epithelial cells.

Methods: Human gingival cells (Ca9-22) were cultured in bacterial extracts prepared from A. actinomycetemcomitans ATCC 29522. The cells were pretreated with protease inhibitors or transfected with small interfering RNA (siRNA) specific for protease-activated receptor 2 (PAR-2).

Results: The pretreatment of cells with serine protease inhibitors significantly inhibited A. actinomycetemcomitans extract-induced expression of interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) at both the messenger RNA and protein levels. In addition, A. actinomycetemcomitans extract-induced IL-8 and ICAM-1 expression was significantly decreased in PAR-2/siRNA-transfected cells.

Conclusions: A. actinomycetemcomitans extract-induced IL-8 and ICAM-1 expression in gingival epithelial cells is mediated by PAR-2.

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蛋白酶激活受体2介导白细胞介素-8和细胞间粘附分子-1表达对聚集杆菌放线菌病的反应。

简介:我们研究了放线菌聚集菌提取物影响牙龈上皮细胞炎症反应的机制。方法:用放线菌ATCC 29522提取液培养人牙龈细胞(Ca9-22)。用蛋白酶抑制剂预处理细胞或用蛋白酶激活受体2 (PAR-2)特异性的小干扰RNA (siRNA)转染细胞。结果:丝氨酸蛋白酶抑制剂预处理细胞可显著抑制放线菌提取物诱导的白细胞介素-8 (IL-8)和细胞间粘附分子-1 (ICAM-1)在信使RNA和蛋白水平的表达。此外，在PAR-2/ sirna转染的细胞中，放线菌提取物诱导的IL-8和ICAM-1表达显著降低。结论:放线菌提取物诱导牙龈上皮细胞IL-8和ICAM-1的表达是由PAR-2介导的。

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Oral microbiology and immunology

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