Dose-dependent effects of triiodothyronine on the osteogenic differentiation of rat bone marrow mesenchymal stem cells.

Hormone research Pub Date : 2009-01-01 Epub Date: 2009-08-18 DOI:10.1159/000232161

J N Boeloni, N M Ocarino, A B Melo, J F Silva, P Castanheira, A M Goes, R Serakides

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引用次数: 40

Abstract

Background/aims: The aim of this study was to investigate the dose-dependent effects of triiodothyronine (T3) on the osteogenic differentiation of mesenchymal stem cells(MSCs).

Methods: MSCs that express CD73, CD54 (intercellular adhesion molecule-1) and CD90 were cultured in triplicate (1 x 10(5)/well) in osteogenic medium with T3 (1, 10, 10(3) or 10(5) pM) or without T3 (control) for 7, 14 and 21 days. Alkaline phosphatase activity, conversion of MTT into formazan crystals, collagen synthesis, collagen maturation, the number of mineralized nodules and their diameters were all determined, and the means were compared by the Student-Newman-Keuls test.

Results: A dose of 10(5) pM T3 resulted in a negative effect on MSC osteogenic differentiation, with less collagen synthesis. The 1 pM T3 dose resulted in greater collagen synthesis and alkaline phosphatase activity and more mineralized nodules than in the control group, similar to the 10 pM dose. Nevertheless, the 10 pM dose demonstrated better results than the 1 pM dose with regard to MSC osteogenic differentiation, with greater MTT reduction, better collagen maturation and a larger mean diameter of mineralized nodules.

Conclusions: The effect of T3 on MSC differentiation is dose-dependent, with the 10 pM dose promoting better bone marrow MSC osteogenic differentiation.

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三碘甲状腺原氨酸对大鼠骨髓间充质干细胞成骨分化的剂量依赖性影响。

背景/目的:本研究旨在探讨三碘甲状腺原氨酸(T3)对间充质干细胞(MSCs)成骨分化的剂量依赖性作用。方法:表达CD73、CD54(细胞间黏附分子-1)和CD90的MSCs在含T3(1,10,10(3)或10(5)pM)或不含T3(对照)的成骨培养基中分三次(1 × 10(5)/孔)培养7、14和21天。测定碱性磷酸酶活性、MTT转化为甲醛晶体、胶原合成、胶原成熟、矿化结节数量及其直径，并通过Student-Newman-Keuls检验比较平均值。结果:10(5)pM T3对MSC成骨分化有负面影响，胶原合成减少。与对照组相比，1 pM T3剂量导致胶原合成和碱性磷酸酶活性增加，矿化结节增多，与10 pM剂量相似。然而，在MSC成骨分化方面，10 pM剂量比1 pM剂量表现出更好的结果，MTT降低更大，胶原成熟更好，矿化结节的平均直径更大。结论:T3对骨髓间充质干细胞分化的影响是剂量依赖性的，10 pM剂量可促进骨髓间充质干细胞更好的成骨分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Hormone research 医学-内分泌学与代谢

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