Interaction between tetraplex structure of mouse telomeric DNA and telomeric DNA binding domains of mouse telomere binding protein Pot1.

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp123

Kaoru Kaneda, Hidetaka Torigoe

引用次数: 1

Abstract

Mouse telomeric DNA sequence, Tel3.5: 5'-AGGG(T TAGGG)(3)-3', has the ability to form antiparallel tetraplex structure in the presence of Na(+). We examined the interaction between the antiparallel tetraplex structure of Tel3.5 and each of two single-stranded telomeric DNA-binding domains of mouse telomere binding protein Pot1, mPot1OB1 and mPot1OB2. The antiparallel tetraplex of Tel3.5 became unfolded upon the interaction with mPot1OB1. On the other hand, no significant structural change of the antiparallel tetraplex of Tel3.5 was observed upon the interaction with mPot1OB2. Considering that the antiparallel tetraplex inhibits telomerase-mediated telomere elongation, we conclude that the ability of mPot1OB1 to unfold the antiparallel tetraplex of the mouse telomeric DNA is required for telomerase-mediated telomere elongation.

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小鼠端粒DNA四复体结构与端粒结合蛋白Pot1端粒DNA结合域的相互作用。

小鼠端粒DNA序列Tel3.5: 5′-AGGG(T TAGGG)(3)-3′在Na(+)存在下能够形成反平行四联体结构。我们研究了Tel3.5的反平行四联体结构与小鼠端粒结合蛋白Pot1、mPot1OB1和mPot1OB2的两个单链端粒dna结合域之间的相互作用。与mPot1OB1相互作用后，Tel3.5的反平行四联体展开。另一方面，与mPot1OB2相互作用后，未观察到Tel3.5的反平行四联体结构发生明显变化。考虑到反平行四联体抑制端粒酶介导的端粒延伸，我们得出结论，mPot1OB1展开小鼠端粒DNA的反平行四联体的能力是端粒酶介导的端粒延伸所必需的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nucleic acids symposium series (2004)

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