Identification of DNA binding specificity for TLS.

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp124

Kentaro Takahama, Shigeki Arai, Riki Kurokawa, Takanori Oyoshi

引用次数: 10

Abstract

TLS (Translocated in liposarcoma) has been characterized as a rearranged gene in chromosomal translocations specific of human myxoid liposarcoma. The various cellular functions of TLS participating either in transcription or splicing processes are thought the involvement of an interaction of TLS with DNA and/or RNA. To investigate insight into DNA-TLS interaction, we performed Electrophoretic mobility shift assay of TLS with G-quadruplex DNA. It revealed that TLS especially bound to single stranded human telomeric DNA in the presence of potassium ion while it was not able to bind double stranded human telomeric DNA and single stranded human telomeric DNA in the presence of sodium ion.

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TLS DNA结合特异性的鉴定。

TLS(易位于脂肪肉瘤)被认为是人黏液样脂肪肉瘤特异性染色体易位中的重排基因。TLS参与转录或剪接过程的各种细胞功能被认为涉及与DNA和/或RNA的相互作用。为了深入了解DNA-TLS的相互作用，我们对TLS与g -四重体DNA进行了电泳迁移转移试验。结果表明，在钾离子存在下，TLS能与单链人端粒DNA结合，而在钠离子存在下，TLS不能与双链人端粒DNA和单链人端粒DNA结合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nucleic acids symposium series (2004)

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