Influence of 3'-azido-2',3'-dideoxyguanosine treatment on telomere length in human telomerase-immortalized human fibroblast cells.

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp125

Yuichi Kurashina, Xiaohong Liu, Chiemi Kato, Naoki Inoue, Mineo Saneyoshi, Toyofumi Yamaguchi

引用次数: 0

Abstract

In order to study telomerase activation in normal cells, a telomerase-immortalized fibroblast cell line, hTERT-BJ1, treated with a telomerase inhibitor, 3'-azido-2',3'-dideoxyguanosine (AZddG), is considered to be a good model. Long-term treatment with AZddG resulted in telomere shortening from 10-20 kbp to 5-6 kbp in cultured hTERT BJ1 cells. However, the telomere length then stabilized. As expected, removal of AZddG from the culture medium induced telomere lengthening in the cells, suggesting that telomerase activity was recovered upon AZddG removal. The effect of recovery of telomerase activity in hTERT-BJ1 cells will be investigated in future studies.

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3′-叠氮-2′，3′-二脱氧鸟苷处理对端粒酶永生化人成纤维细胞端粒长度的影响。

为了研究正常细胞中端粒酶的活化，端粒酶永活的成纤维细胞系hTERT-BJ1被认为是一个很好的模型，该模型使用端粒酶抑制剂3'-叠氮-2'，3'-双脱氧鸟苷(AZddG)处理。AZddG长期治疗可使培养的hTERT BJ1细胞端粒从10-20 kbp缩短至5-6 kbp。然而，端粒长度随后趋于稳定。不出所料，从培养基中去除AZddG诱导细胞端粒延长，表明去除AZddG后端粒酶活性恢复。端粒酶活性恢复对hTERT-BJ1细胞的影响将在未来的研究中进一步探讨。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nucleic acids symposium series (2004)

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