Is lysyl oxidase-like protein-1, alpha-1 antitrypsin, and neutrophil elastase site specific in pelvic organ prolapse?

Weng Chi Man, Jason Yen-Ping Ho, Yan Wen, Eric R Sokol, Mary L Polan, Bertha Chen
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引用次数: 10

Abstract

Introduction and hypothesis: We investigated whether the expression of alpha-1 antitrypsin (ATT), neutrophil elastase (NE), and lysyl oxidase-like protein 1 (LOXL-1) vary within the vagina in subjects with pelvic organ prolapse (POP).

Methods: Biopsies were obtained from the anterior and posterior vaginal wall of 22 women with POP (> or =stage 2 by POP-Q). The subjects were grouped by the most prominent defect: cystocele, cystocele plus uterine prolapse, and rectocele. Comparative real-time PCR, Western blotting, and NE enzyme activity assay were performed.

Results: The ratio of anterior and posterior vaginal wall ATT, NE, and LOXL-1 expression varied between individuals within the same defect group.

Conclusions: ATT, NE, and LOXl-1 expression was variable among different biopsy sites in the vagina. No consistent pattern was present when the subjects were grouped by the most prominent defect. We recommend careful consideration of biopsy sites in future studies on POP to enhance reproducibility of data.

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赖氨酸氧化酶样蛋白-1、α -1抗胰蛋白酶和中性粒细胞弹性蛋白酶位点在盆腔器官脱垂中特异性吗?
前言和假设:我们研究了α -1抗胰蛋白酶(ATT)、中性粒细胞弹性蛋白酶(NE)和赖氨酸氧化酶样蛋白1 (LOXL-1)在盆腔器官脱垂(POP)患者阴道内的表达是否存在差异。方法:对22例POP(>或= 2期POP- q)患者阴道前后壁行活检。根据最突出的缺陷进行分组:膀胱膨出、膀胱膨出合并子宫脱垂和直肠膨出。进行实时荧光定量PCR、Western blotting和NE酶活性测定。结果:阴道前后壁ATT、NE、LOXL-1的表达比例在同一缺损组个体间存在差异。结论:ATT、NE和LOXl-1在阴道不同活检部位的表达是不同的。当受试者按最突出的缺陷分组时,没有一致的模式出现。我们建议在未来的POP研究中仔细考虑活检部位,以提高数据的可重复性。
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