Metal ion binding of modified pyrimidine-base pairs in DNA duplexes.

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp009

Akira Ono, Kenji Iwamoto, Kumiko Sugiyama, Itaru Okamoto

引用次数: 0

Abstract

We report the synthesis and metal ion-binding properties of DNA duplexes containing 5-substituted uracil ((X)U) pairs, such as 5-bromouracil, 5-fluorouracil and 5-cyanouracil pairs. Thermal denaturation studies of these modified DNA duplexes revealed that the DNA duplexes were stabilized in the presence of Hg(II) ions in acidic and neutral solutions. On the other hand, the duplexes were stabilized in the presence of Hg(II) and Ag(I) ions. These results indicated that (X)U-Hg(II)-(X)U complex was formed in the acidic and neutral solutions, then, in the basic solutions (X)U-Ag(I)-(X)U complex as well as the (X)U-Hg(II)-(X)U complex were formed. ESI-TOF MS analysis also indicated formation of the metal ion-DNA complexes.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

DNA双链中修饰嘧啶碱基对的金属离子结合。

我们报道了含有5-取代尿嘧啶((X)U)对的DNA双链的合成和金属离子结合性质，如5-溴脲嘧啶、5-氟尿嘧啶和5-氰脲嘧啶对。对这些修饰的DNA双链的热变性研究表明，DNA双链在酸性和中性溶液中在Hg(II)离子存在下是稳定的。另一方面，双相化合物在Hg(II)和Ag(I)离子存在下稳定。结果表明，(X)U- hg (II)-(X)U配合物首先在酸性溶液和中性溶液中形成，然后在碱性溶液中形成(X)U- ag (I)-(X)U配合物和(X)U- hg (II)-(X)U配合物。ESI-TOF MS分析也显示了金属离子- dna络合物的形成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Nucleic acids symposium series (2004)

自引率

0.00%

发文量

期刊最新文献

Chemical methods to study protein-nucleic acid interactions. Development of novel chemical probes to detect abasic sites in DNA. Assessment of the DNA damage using the fluorescence microscope. Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe. Multiple activities of c-di-GMP in Pseudomonas aeruginosa.