Evaluation of mobility of 2-N-tert-butylaminoxyladenosine incorporated into oligodeoxynucleotides.

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI:10.1093/nass/nrp076

Yoshitaka Higuchi, Mariko Aso, Ryouko Harada, Noboru Koga, Hiroshi Suemune

引用次数: 0

Abstract

We synthesized oligodeoxynucleotide (ODN, 3) containing 2-N-tert-butylaminoxyladenosine (1) and studied EPR spectra of 3 and its duplexes. The h(+)/h(0) values in the EPR spectra of duplexes between 3 and 5-8 well correlated with Tm values. We also synthesized ODN 4 containing 2, which has a cyclic aminoxyl via a linker, to compare with ODN 3. The h(+)/h(0) values in the EPR spectra of duplexes between 4 and 5-8 did not correlate with Tm values. These results indicate that 1 has a potential to monitor of motion of the nucleobase.

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低聚脱氧核苷酸中2- n -叔丁基氨基氧化腺苷迁移率的评价。

合成了含有2- n -叔丁基氨基氧化腺苷(1)的寡脱氧核苷酸(ODN, 3)，并研究了3及其双链物的EPR谱。在3 ~ 5-8之间的双相化合物的EPR光谱中的h(+)/h(0)值与Tm值有很好的相关性。我们还合成了含有2的odn4，它通过一个连接体有一个环氨基氧基，与odn3比较。在4 ~ 5-8之间的双相化合物的EPR光谱中的h(+)/h(0)值与Tm值不相关。这些结果表明，1具有监测核碱基运动的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nucleic acids symposium series (2004)

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