The three-dimensional structure of Infectious flacherie virus capsid determined by cryo-electron microscopy.

Science in China. Series C, Life Sciences / Chinese Academy of Sciences Pub Date : 2009-12-01 Epub Date: 2009-12-17 DOI:10.1007/s11427-009-0151-z

Li Xie, QinFen Zhang, XingMeng Lu, XinHong Dai, KunPeng Li, Jian Hong, XuePing Zhou

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引用次数: 5

Abstract

Cryo-electron microscopy and image reconstruction were used to determine the three-dimensional structure of Infectious flacherie virus (IFV). 5047 particles were selected for the final reconstruction. The FSC curve showed that the resolution of this capsid structure was 18 A. The structure is a psuedo T=3 (P=3) icosahedral capsid with a diameter of 302.4 A and a single shell thickness of 15 A. The density map showed that IFV has a smooth surface without any prominent protrude or depression. Comparison of the IFV structure with those of the insect picorna-like virus-Cricket paralysis virus (CrPV)and human picornavirus-Human rhinovirus 14 (HRV 14) revealed that the IFV structure resembles the CrPV structure. The "Rossmann canyon" is absent in both IFV and CrPV particles. The polypeptide topology of IFV VP2, IFV VP3 was predicted and the subunit location at the capsid surface was further analyzed.

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用低温电子显微镜测定感染性裂孔病毒衣壳的三维结构。

采用低温电子显微镜和图像重建技术确定了感染性裂口病毒(IFV)的三维结构。最终选择5047个粒子进行重建。FSC曲线显示该衣壳结构的分辨率为18 A。该结构为伪T=3 (P=3)二十面体衣壳，其直径为302.4 a，单壳厚度为15 a。密度图显示IFV表面光滑，无明显的凸起或凹陷。与昆虫小核糖核酸样病毒-蟋蟀麻痹病毒(CrPV)和人小核糖核酸病毒-人鼻病毒14 (HRV 14)的结构比较发现，IFV的结构与CrPV的结构相似。在IFV和CrPV粒子中均不存在“罗斯曼峡谷”。预测了IFV VP2、IFV VP3的多肽拓扑结构，并进一步分析了其在衣壳表面的亚基位置。

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Science in China. Series C, Life Sciences / Chinese Academy of Sciences

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