Wei-dong Wu, Chi-hua Fang, Zheng-xin Yang, Jia-jin Bao
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引用次数: 0
Abstract
Objective: To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells.
Methods: Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR.
Results: After transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05).
Conclusion: Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.
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