Quantification of norepinephrine and its metabolites in the plasma of renal failure models.

Nephron Physiology Pub Date : 2010-01-01 Epub Date: 2010-07-08 DOI:10.1159/000318177

Hiroshi Iijima, Yuji Okada, Makoto Tsunoda, Tomoko Takamiya, Kazuhiro Imai

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引用次数: 2

Abstract

Background/aims: The plasma concentration of catecholamines and their metabolites generated by catechol-O-methyl transferase (COMT) were measured and their correlation with the progress of renal dysfunction was investigated in two distinctive animal models: a 5/6 nephrectomized Sprague-Dawley rat model and a 1/2 nephrectomized diabetic fatty Zucker rat model.

Methods: A highly sensitive, high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection was employed to obtain values for the ratio [NMN]/([NE] + [NMN]), where [NE] represents the plasma concentration of norepinephrine and [NMN] represents the plasma concentration of normetanephrine.

Results: The [NMN]/([NE] + [NMN]) ratio correlated with both the increase in blood urea nitrogen concentration and the decrease in creatinine clearance.

Conclusion: The [NMN]/([NE] + [NMN]) ratio represents a quantitative indicator of the progress of renal dysfunction in the animal models. Regulation of COMT activity seemed to relate with the progress of renal dysfunction.

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肾衰模型血浆中去甲肾上腺素及其代谢物的定量测定。

背景/目的:采用5/6肾切除的Sprague-Dawley大鼠模型和1/2肾切除的糖尿病脂肪性大鼠模型，测定血浆中儿茶酚胺及其代谢物(COMT)的浓度，并探讨其与肾功能障碍进展的相关性。方法:采用高灵敏度、高效液相色谱-过氧草酸盐化学发光反应检测法获得比值[NMN]/([NE] + [NMN])，其中[NE]代表血浆去甲肾上腺素浓度，[NMN]代表血浆去甲肾上腺素浓度。结果:[NMN]/([NE] + [NMN])比值与血尿素氮浓度升高和肌酐清除率降低相关。结论:[NMN]/([NE] + [NMN])比值是动物模型肾功能损害进展的定量指标。COMT活性的调节似乎与肾功能不全的进展有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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