Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristata DC), its extract, and in commercially marketed Ayurvedic dosage forms.

International journal of Ayurveda research Pub Date : 2010-10-01 DOI:10.4103/0974-7788.76789

Anubhuti Pasrija, Rahul Singh, Chandra Kant Katiyar

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引用次数: 32

Abstract

A new, simple, sensitive, selective, and precise high-performance liquid chromatography (HPLC) method for analysis of berberine in crude plant material, herbal extract, and ayurvedic dosage forms was developed and validated. The stationary phase was inert sil C(18) column The mobile phase consisting of acetonitrile (HPLC Grade) and potassium dihydrogen phosphate buffer (pH 2.5) in a gradient flow was used.The column was equilibrated with the mobile phase (flow rate 1.0 ml/min); elution was monitored at 346 nm. The linear regression analysis data for the calibration plots showed good linear relationship, with r(2)= 0.9942 in the concentration range of 16380-30420 μg/ml with respect to the peak area. The method was validated for specificity, precision, recovery, and linearity according to the International Conference on Harmonization guidelines. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of berberine.

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验证了HPLC-UV法测定天然草药小檗(Berberis aristata DC)及其提取物和市售阿育吠陀剂型中小檗碱的含量。

建立了一种简便、灵敏、选择性和精确的高效液相色谱(HPLC)方法，用于分析植物原料、草药提取物和阿育吠陀剂型中的小檗碱。固定相为C(18)柱，流动相为乙腈(HPLC级)和磷酸二氢钾缓冲液(pH 2.5)，梯度流动。用流动相(流速1.0 ml/min)平衡柱;在346 nm处监测洗脱。线性回归分析结果表明，在16380 ~ 30420 μg/ml浓度范围内，峰面积r(2)= 0.9942，线性关系良好。根据国际协调会议的指导方针，验证了该方法的特异性、精密度、回收率和线性。统计分析结果表明，该方法对小檗碱的测定具有良好的重复性和选择性。

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International journal of Ayurveda research

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