Development of an HPLC method for determination of pentachloronitrobenzene, hexachlorobenzene and their possible metabolites.

BMC chemical biology Pub Date : 2011-11-23 DOI:10.1186/1472-6769-11-2

Fazlurrahman Khan, Dhan Prakash, Rk Jain

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引用次数: 8

Abstract

Background: Pentachloronitrobenzene (PCNB) and hexachlorobenzene (HCB) are highly toxic and widespread in every environmental compartment. Some of metabolic products such as amino/nitro containing chlorinated aromatic compounds can be determined by gas chromatography coupled with electron capture detector (GC-ECD). However, it is difficult to identify some of chlorophenolic and chloroquinolic intermediates produced from PCNB and HCB by the above mentioned technique. Therefore, for analysis of these compounds and their metabolites, we have developed a high performance liquid chromatography (HPLC) based method.

Results: The extraction of PCNB and HCB from soil and minimal salt medium was carried out with ethyl acetate and hexane respectively with good recoveries (98% for PCNB and 97% for HCB). The validation of the proposed extraction and HPLC method was done by analysis of PCNB and HCB biodegradation and their metabolites identification from anaerobic enriched soil samples.

Conclusion: A rapid, sensitive and simple HPLC based analytical method was developed for the analysis of PCNB, HCB and their possible intermediates.

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建立HPLC法测定五氯硝基苯、六氯苯及其可能代谢物的方法。

背景:五氯硝基苯(PCNB)和六氯苯(HCB)具有剧毒，广泛存在于各个环境隔间中。气相色谱联用电子捕获检测器(GC-ECD)可以测定一些代谢产物，如含氯代芳香化合物的氨基/硝基化合物。但是，用上述方法鉴定PCNB和HCB所产生的一些氯酚类和氯喹啉类中间体存在一定的困难。因此，为了分析这些化合物及其代谢物，我们开发了一种基于高效液相色谱(HPLC)的方法。结果:用乙酸乙酯和己烷分别从土壤和微量盐培养基中提取PCNB和HCB，回收率为98%和97%。通过分析富氧土壤样品中PCNB和HCB的生物降解及其代谢物鉴定，验证了所提出的提取和HPLC方法。结论:建立了一种快速、灵敏、简便的PCNB、HCB及其可能中间体的高效液相色谱分析方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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BMC chemical biology

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