[Study of antibiotic resistance of enterococci strains received in 2009 in the National Reference Centre for Enterococci].

Mihaela-Cristin Giucă, Anca Petrini, Tuca Ceornea, Vasilica Ungureanu
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Abstract

Unlabelled: Enterococci are a frequent cause of a variety of human infections, the most common being urinary tract infection followed by bacteraemia, meningitis and endocarditis from hospitalized patients.

Objective: The study and monitoring of antibiotic resistance of enterococci strains isolated and confirmed in 2009.

Materials and methods: Identification of 30 strains received in 2009 was based on phenotypic characteristics (microscopy, culture and biochemical characters); antibiotic susceptibility testing was performed according to CLSI standards 2009 by diffusion test and MIC by agar dilution and E-test.

Result: The strains belonged to the following species: E. faecalis (18), E. faecium (6), E. gallinarum (5), and E. durans (1), and were isolated from: blood cultures, urine, pus-wound, cerebrospinal fluid (CSF) and catheter. Antibiotic susceptibility testing showed susceptibility to different antibiotics depending on the species and also on the type of clinical samples.

Conclusions: Due to the small number of strains, no resistance phenotypes could be determined. As emergence of antibiotic resistant enterococci is well known, continuous surveillance of antibiotic susceptibility and molecular study of this issue are required.

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[2009年国家肠球菌参考中心接收的肠球菌菌株抗生素耐药性研究]。
未标记:肠球菌是多种人类感染的常见原因,最常见的是尿路感染,其次是住院患者的菌血症、脑膜炎和心内膜炎。目的:对2009年分离确诊肠球菌的耐药情况进行研究和监测。材料与方法:2009年收到的30株菌株根据表型特征(显微镜、培养和生化特征)进行鉴定;药敏试验按CLSI标准2009采用扩散试验,MIC采用琼脂稀释法和e试验。结果:菌株属粪肠球菌(18株)、粪肠球菌(6株)、鸡肠球菌(5株)和杜兰肠球菌(1株),分别从血培养物、尿液、伤口、脑脊液和导管中分离得到。抗生素药敏试验显示对不同抗生素的敏感性取决于菌种和临床样本的类型。结论:由于菌株数量少,无法确定耐药表型。由于耐药肠球菌的出现是众所周知的,因此需要对抗生素敏感性进行持续监测并对此问题进行分子研究。
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